Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 Sep;38(1):83-9.
doi: 10.1016/0168-1702(95)00046-s.

Cloning and sequencing of a 8.4-kb region from the 3'-end of a Taiwanese virulent isolate of the coronavirus transmissible gastroenteritis virus

C M Chen  1 , D Cavanagh, P Britton
Affiliations

Cloning and sequencing of a 8.4-kb region from the 3'-end of a Taiwanese virulent isolate of the coronavirus transmissible gastroenteritis virus

C M Chen et al. Virus Res. 1995 Sep.

Abstract

The nucleotide sequence (8396 nucleotides) was determined, from the 3'-end of the putative polymerase gene to the poly(A) tail, for a Taiwanese virulent isolate, TFI, of transmissible gastroenteritis virus (TGEV). The TFI nucleotide sequence had very high identity to the British virulent field isolate FS772/70 (98.3%), the attenuated Purdue 115 (96.7%) and from the S gene to ORF-4 gene region, to the low passaged virulent Miller (98.3%) strains of TGEV. Comparison of the TFI S protein sequence with those determined from other TGEV strains and those of the TGEV variant, porcine respiratory coronavirus, isolated from Europe and North America showed that they had changed very little over a period of 4 decades. The two extra amino acids found to be present in the spike proteins of the virulent FS772/70 and Miller strains when compared to the avirulent Purdue strain were found to be present in the TFI strain. The genomic organisation of the TFI strain was the same as that of the other TGEV viruses.

PubMed Disclaimer

References

    1. Britton P., Page K.W. Sequence of the S gene from a virulent British field isolate of transmissible gastroenteritis virus. Virus Res. 1990;18:71–80. - PMC - PubMed
    1. Britton P., Mawditt K.L., Page K.W. The cloning and sequencing of the virion protein genes from a British isolate of porcine respiratory coronavirus: comparison with transmissible gastroenteritis virus genes. Virus Res. 1991;21:181–198. - PMC - PubMed
    1. Britton P., Kottier S., Chen C.-M., Pocock D.H., Salmon H., Aynaud J.M. The use of PCR genome mapping for the characterisation of TGEV strains. In: Laude H., Vautherot J.F., editors. Coronaviruses: Molecular Biology and Virus-Host Interactions. Vol. 342. Plenum Press; New York: 1993. pp. 29–34. (Adv. Exp. Med. Biol.). - PubMed
    1. Chen C.M., Tien S.C., Wu J.L., Chu R.M. The phenotype of Taiwan field isolated transmissible gastroenteritis virus (TGEV) is different from the other TGEV. J. Chin. Soc. Vet. Sci. 1989;15:11–18.
    1. Correa I., Gebauer F., Bullido M.J., Sune C., Baay M.F.D., Zwaagstra K.A., Posthumus W.P.A., Lenstra J.A., Enjuanes L. Localisation of antigenic sites of the E2 glycoprotein of transmissible gastroenteritis virus. J. Gen. Virol. 1990;71:271–279. - PubMed

Publication types

Substances

Associated data

LinkOut - more resources