[Cloning and expression of the Bacillus stearothermophilus neutral proteinase gene in Bacillus subtilis cells]

Abstract

Gene of thermostable Bacillus stearothermophilus metalloprotease was cloned and expressed in mesophilic Bacillus subtilis cells. It was demonstrated that this gene is closely related by its restriction map to B. stearothermophilus T metalloprotease gene cloned earlier. Thermostability level and thermal optimum of activity of metalloprotease, the product of cloned gene expression, were estimated.