[Incorporation of 1-14C-glycine into total protein and subcellular fractions of rat liver at different periods following administration of tetrachlormethane]

Abstract

Rate of incorporation of 1-14C-glycine in total protein and subcellular fractions of rat liver tissue as well as the activity of alanine- and aspartate aminotransferases in blood serum were studied at various periods after treatment with CCl4. The protein synthesis was distinctly decreased in liver tissue and the alanine aminotransferase activity was markedly increased in blood serum with the first days after CCl4 administration. Dissimilar alterations were observed in the rate of the label incorporation into nuclear and mitochondrial fractions after prolonged administration of CCl4. Hepatocyte nuclei proved to be more sensitive to cytoplasmic alterations caused by tetrachlormethane. Incorporation of 1-14C-glycine into both nuclear and mitochondrial fractions was decreased only at later periods. In blood serum the alanine aminotransferase activity was drastically increased after prolonged administration of CCl4, whereas the aspartate aminotransferase activity was increased as compared with control less markedly.