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. 1995 Dec;29(5):897-907.
doi: 10.1007/BF00014964.

Transformation of Synechococcus with a gene for choline oxidase enhances tolerance to salt stress

P Deshnium  1 , D A Los, H Hayashi, L Mustardy, N Murata
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Transformation of Synechococcus with a gene for choline oxidase enhances tolerance to salt stress

P Deshnium et al. Plant Mol Biol. 1995 Dec.

Abstract

Choline oxidase, isolated from the soil bacterium Arthrobacter globiformis, converts choline to glycinebetaine (N-trimethylglycine) without a requirement for any cofactors. The gene for this enzyme, designated codA, was cloned and introduced into the cyanobacterium Synechococcus sp. PCC 7942. The codA gene was expressed under the control of a strong constitutive promoter, and the transformed cells accumulated glycinebetaine at intracellular levels of 60-80 mM. Consequently the cells acquired tolerance to salt stress, as evaluated in terms of growth, accumulation of chlorophyll and photosynthetic activity.

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References

    1. FEBS Lett. 1992 Jan 20;296(2):187-9 - PubMed
    1. Biochim Biophys Acta. 1990 Jun 20;1034(3):253-9 - PubMed
    1. Biochem Biophys Res Commun. 1979 Feb 28;86(4):1084-8 - PubMed
    1. Mol Microbiol. 1991 May;5(5):1049-64 - PubMed
    1. Plant Physiol. 1986 Aug;81(4):1044-9 - PubMed

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