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. 1996 Jan 15;61(1):1-4.
doi: 10.1097/00007890-199601150-00001.

Multilineage hematopoietic reconstitution of supralethally irradiated rats by syngeneic whole organ transplantation. With oarticular reference to the liver

N Murase  1 , T E Starzl, Q Ye, A Tsamandas, A W Thomson, A S Rao, A J Demetris
Affiliations

Multilineage hematopoietic reconstitution of supralethally irradiated rats by syngeneic whole organ transplantation. With oarticular reference to the liver

N Murase et al. Transplantation. .
No abstract available

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Figures

Figure 1
Figure 1
Total white cell counts (mm3) in normal and irradiated LEW rats (9.5 Gy) and rats given syngeneic transplants, after treatment with red blood cell lysing buffer. Cells were counted in a hemacytometer. ●, Normal Lewis (n=3); ○, radiation alone (n=3); ■, 1×106 bone marrow (n=6); □, 10×106 bone marrow (n=4); ▲, OLTX (n=3).
Figure 2
Figure 2
Hematocrit (tail vein blood, microcentrifuge method) in same samples as Figure 1.
Figure 3
Figure 3
Body weight changes in animals of Figures 1 and 2. ●, Normal LEW (n=3); ○, radiation alone (n=3); ■ 1×106 bone marrow (n=6); □, 10×106 bone marrow (n=4); ▲ OLTx (n=3).
Figure 4
Figure 4
Lineage of peripheral leukocytes shown in Figure 1. Granulocytes were determined on blood smear following α-naphthyl acetate esterase and hematoxylin staining. Other FACS analysis of percentage of subpopulations (mean ± SD) was by direct immunofluorescence with antibodies to surface markers, including OX1 (CD45), R7.3 (αβ TCR), W3/25 (CD4), OX8 (CD8), OX33 (B-cells), and NK3.2.3 (NK cells).
Figure 5
Figure 5
Polymerase chain reaction and Southern hybridization of organs and tissues 50 days after lethal irradiation and syngeneic liver transplantation. PBL, peripheral blood lymphocytes; Ty, thymus; SP, spleen; BM, bone marrow; CLN, cervical lymph nodes. Male and female (negative) controls are included.
See this image and copyright information in PMC

References

    1. Starzl TE, Demetris AJ, Murase N, Ildstad S, Ricordi C, Trucco M. Cell migration, chimerism, and graft acceptance. Lancet. 1992;339:1579. - PMC - PubMed
    1. Starzl TE, Demetris AJ, Trucco M, et al. Cell migration and chimerism after whole-organ transplantation: the basis of graft acceptance. Hepatology. 1993;17:1127. - PMC - PubMed
    1. Iwaki Y, Starzl TE, Yagihashi A, et al. Replacement of donor lymphoid tissue in human small bowel transplants under FK 506 immunosuppression. Lancet. 1991;337:818. - PMC - PubMed
    1. Murase N, Demetris AJ, Woo J, et al. Graft versus host disease (GVHD) after BN to LEW compared to LEW to BN rat intestinal transplantation under FK 506. Transplantation. 1993;55:1. - PMC - PubMed
    1. Murase N, Starzl TE, Tanabe M, et al. Variable chimerism, graft versus host disease, and tolerance after different kinds of cell and solid organ transplantation from Lewis to Brown-Norway rats. Transplantation. 1995;60:158. - PMC - PubMed

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