The recA gene of Borrelia burgdorferi

Abstract

The nucleotide sequence of the Borrelia burgdorferi (Bb) Sh-2-82 recA gene has been determined using PCR-based approaches without the construction of a genomic library. The gene should encode a protein of 365 amino acids which is highly homologous to other known RecA proteins. It represents a new homolog from a distinct phylogenetic branch of eubacteria. Although, previous reports concluded that recA is absent from Bb, the identification presented here conclusively shows its presence and reaffirms the ubiquity of RecA in prokaryotes.