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. 1995 Dec 29;167(1-2):335-6.
doi: 10.1016/0378-1119(95)00652-4.

Antibiotic-resistance cassettes for Bacillus subtilis

A M Guérout-Fleury  1 , K Shazand, N Frandsen, P Stragier
Affiliations

Antibiotic-resistance cassettes for Bacillus subtilis

A M Guérout-Fleury et al. Gene. .

Abstract

The genes encoding resistance to four different antibiotics (erythromycin, kanamycin, tetracycline and spectinomycin) were cloned in the polylinker of various Escherichia coli plasmid vectors. These cassettes can be inserted into cloned Bacillus subtilis (Bs) genes and used to create tagged chromosomal disruptions after recombination into Bs and selection in the presence of the appropriate antibiotic.

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