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. 1977 Jan;13(1):1-10.
doi: 10.1007/BF02615497.

Critical adjustment of cysteine and glutamine concentrations for improved clonal growth of WI-38 cells

R G Ham, S L Hammond, L L Miller

Critical adjustment of cysteine and glutamine concentrations for improved clonal growth of WI-38 cells

R G Ham et al. In Vitro. 1977 Jan.

Abstract

Clonal growth of WI-38 cells with a plating efficiency of 45% has been achieved in a synthetic nutrient mixture (MCDB 102) supplemented with either whole or dialyzed fetal bovine serum precisely. Deviation by a factor of three in either direction from the optimum concentration (9.0 x 10(-5) M) eliminates essentially all clonal growth. A high concentration of glutamine (2.5 x 10(-3) M) is also needed for optimum clonal growth.

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