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. 1977 Apr 25;252(8):2584-91.

Chemical structure of the galactomannan from the cell wall of Aspergillus niger

  • PMID: 856795
Free article

Chemical structure of the galactomannan from the cell wall of Aspergillus niger

P C Bardalaye et al. J Biol Chem. .
Free article

Abstract

The galactomannan of surface grown Aspergillus niger has been isolated by alkaline extraction of hyphal walls and characterized structurally. Its elution profile, from a column of Bio-Gel P-150, reveals a broad range of molecular sizes grouped into two fractions. Gas chromatographic and colorimetric analyses indicate that each fraction is composed of approximately equimolar quantities of galactose and mannose plus 12 to 14% glucose. Both have similar low optical rotations and contain acid labile galactose. Methylation, Smith degradation, acetolysis, reactivity with concanavalin A and beta-D-galactofuranosidase, plus digestionof galactose with D-galactose oxidase, were techniques employed to determine the polysaccharide's covalent structure. Results of these studies indicate that it is composed of a series of chains, 5 to 9 hexose units in length, connected by alpha1 leads to 6 bonds between mannopyranosyl moieties. The external portion of each chain consists of a galactose tri- or tetrasaccharide of the general structure Galf beta1 leads to 4 Galp(1-2) 1 leads to 4 Galp1 leads to. This segment is connected via a (1 leads to 2) linkage to the internal portion which is a di- to pentasaccharide of mannopyranosyl units joined in alpha1 leads to 2 glycoside linkage. Combination mild acid hydrolysis and methylation experiments indicate galactofuranosyl terminal units are attached only to galactose. The organization of glucose into the overall structure of the polymer has not been determined. Structural relationships of this polysaccharide to both fungal and yeast galactomannans are discussed.

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