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. 1995 Oct;9(5):287-90.
doi: 10.1016/s0890-8508(95)91508-7.

Detection and genotyping of herpes simplex virus types 1 and 2 by polymerase chain reaction

G Lucotte  1 , C Bathelier, V Lespiaux, C Bali, T Champenois
Affiliations

Detection and genotyping of herpes simplex virus types 1 and 2 by polymerase chain reaction

G Lucotte et al. Mol Cell Probes. 1995 Oct.

Abstract

A simple and rapid polymerase chain reaction (PCR) procedure was developed for simultaneous detection and typing of herpes simplex virus (HSV) types 1 and 2. It was possible to detect and type HSV using two primers pairs in a simultaneous double PCR reaction, where the type of HSV present was determined on the basis of an ethidium-bromide-stained band after agarose gel electrophoresis. This PCR assay was tested on about 500 clinical specimens.

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