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. 1996 Jan 23;93(2):780-4.
doi: 10.1073/pnas.93.2.780.

Z-DNA-forming sites within the human beta-globin gene cluster

V Müller  1 , M Takeya, S Brendel, B Wittig, A Rich
Affiliations

Z-DNA-forming sites within the human beta-globin gene cluster

V Müller et al. Proc Natl Acad Sci U S A. .

Abstract

Agarose-encapsulated, metabolically active, permeabilized nuclei from human hematopoietic cell lines were tested for Z-DNA formation in the beta-globin gene cluster. Biotinylated monoclonal antibodies against Z-DNA were diffused into the nuclei and cross-linked to DNA with a 10-ns laser exposure at 266 nm. Following digestion with restriction enzymes, fragments that had formed Z-DNA were isolated. Seventeen regions with Z-DNA sequence motifs in the 73-kb region were studied by PCR amplification, and five were found in the Z conformation.

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