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. 1996 Jan;62(1):279-82.
doi: 10.1128/aem.62.1.279-282.1996.

Site-directed mutations in the third domain of Bacillus thuringiensis delta-endotoxin CryIAa affect its ability to increase the permeability of Bombyx mori midgut brush border membrane vesicles

M G Wolfersberger  1 , X J Chen, D H Dean
Affiliations

Site-directed mutations in the third domain of Bacillus thuringiensis delta-endotoxin CryIAa affect its ability to increase the permeability of Bombyx mori midgut brush border membrane vesicles

M G Wolfersberger et al. Appl Environ Microbiol. 1996 Jan.

Abstract

A series of mutant Bacillus thuringiensis CryIAa delta-endotoxin proteins was prepared by replacing the first, second, and last arginine residues of the conserved third-domain sequence, R-521 YRVRIR-527, with other amino acids. The stable mutant proteins were bioassayed against Bombyx mori larvae and found to all be approximately half as active as wild-type CryIAa. The toxins were also tested by means of a light-scattering assay for their ability to increase permeability of larval B. mori midgut brush border membrane vesicles. Three of the mutant toxins were as active as the wild-type toxin in the vesicle permeability assay.

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References

    1. J Exp Biol. 1979 Dec;83:293-304 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Jun;86(11):4037-41 - PubMed
    1. Microbiol Rev. 1989 Jun;53(2):242-55 - PubMed
    1. Eur J Biochem. 1989 Dec 8;186(1-2):239-47 - PubMed

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