Long-chain diglycerol tetraethers from Thermoplasma acidophilum

Abstract

The C40 isopranol-containing glycerol ether residues which characterize the complex lipids of the extreme thermoacidophile Thermoplasma acidophilum were isolated and purified from the glycolipid and phospholipid fractions. The glycerol ether, as well as the acetate and methoxy derivatives were characterized by thin-layer, gel-permeation and gas-liquid chromatography, infrared, nuclear magnetic resonance and mass spectrometry and by vapor phase osmometry. The glycerol ethers are proposed to be unique fully saturated diglycerol tetraethers, primarily C86H172O6, Mr 1300, which contain two sn-2,3-glycerol residues bridged through ether linkages by two C40 isopranoid branched diols.