Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995;26(5-6):477-85.

The NV genes of fish rhabdoviruses: development of RNase protection assays for rapid assessment of genetic variation

G Kurath  1 , K H Higman, H V Björklund
Affiliations
  • PMID: 8581026

The NV genes of fish rhabdoviruses: development of RNase protection assays for rapid assessment of genetic variation

G Kurath et al. Vet Res. 1995.

Abstract

The genomic RNA of the fish rhabdovirus infectious hematopoietic necrosis virus (IHNV) contains a small NV gene which is not present in any other rhabdoviruses characterized to date. We have constructed a plasmid which carries a full length cDNA copy of the IHNV NV gene between 2 RNA polymerase promoters such that plus-sense and minus-sense RNA transcripts of the IHNV NV gene can be synthesized. These were used to develop an RNase protection assay which was capable of detecting as little as 1-5 ng of NV mRNA or genomic RNA. RNA from tissue culture cells infected with 3 different strains of IHNV showed distinct, diagnostic cleavage patterns reflecting sequence variation in their NV genes.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

  • Viruses of lower vertebrates.
    Essbauer S, Ahne W. Essbauer S, et al. J Vet Med B Infect Dis Vet Public Health. 2001 Aug;48(6):403-75. doi: 10.1046/j.1439-0450.2001.00473.x. J Vet Med B Infect Dis Vet Public Health. 2001. PMID: 11550762 Free PMC article. Review.

MeSH terms