doi: 10.1126/science.271.5251.990.
Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA
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- PMID: 8584937
- DOI: 10.1126/science.271.5251.990
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Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA
Science.
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Abstract
Variants of lambda repressor and cytochrome b562 translated from messenger RNAs without stop codons were modified by carboxyl terminal addition of an ssrA-encoded peptide tag and subsequently degraded by carboxyl terminal-specific proteases present in both the cytoplasm and periplasm of Escherichia coli. The tag appears to be added to the carboxyl terminus of the nascent polypeptide chain by cotranslational switching of the ribosome from the damaged messenger RNA to ssrA RNA.
Comment in
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When proteins receive deadly messages at birth.Science. 1996 Feb 16;271(5251):955-6. doi: 10.1126/science.271.5251.955. Science. 1996. PMID: 8584931 No abstract available.
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