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. 1995 Dec;33(12):3338-40.
doi: 10.1128/jcm.33.12.3338-3340.1995.

Inhibitory effect of sodium dodecyl sulfate in detection of Mycobacterium tuberculosis by amplification of rRNA

J M Manterola  1 , F Gamboa, J Lonca, L Matas, J Ruiz Manzano, C Rodrigo, V Ausina
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Inhibitory effect of sodium dodecyl sulfate in detection of Mycobacterium tuberculosis by amplification of rRNA

J M Manterola et al. J Clin Microbiol. 1995 Dec.

Abstract

The concentrations of sodium dodecyl (lauryl) sulfate (SDS) found in the sediments of the respiratory samples pretreated with SDS-NaOH varied between 3.36 and 12.42 mg/ml. These concentrations of SDS were higher than the level considered critical (> or = 0.16 mg/ml) to obtain negative results with the Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test. Extensive washing, after neutralization of sediment, is sufficient to remove all traces of detergent which interfere with the enzymes used by the Mycobacterium Tuberculosis Direct Test assay.

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