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. 1995 Dec;164(6):444-7.
doi: 10.1007/BF02529743.

Southern blotting analyses of strains of Campylobacter fetus using the conserved region of sapA

M Fujita  1 , T Morooka, S Fujimoto, T Moriya, K Amako
Affiliations

Southern blotting analyses of strains of Campylobacter fetus using the conserved region of sapA

M Fujita et al. Arch Microbiol. 1995 Dec.

Abstract

Chromosomal DNA of 27 strains of Campylobacter fetus was analyzed by Southern blotting with a probe of the conserved region of sapA. The probe hybridized with 23 strains that produced type A lipopolysaccharide. These strains had more than six sapA homologs. In Southern blots of SalI-digested chromosomal DNA separated by pulsed-field gel electrophoresis, one fragment from 19 strains and two fragments from 4 strains hybridized. These data indicate that multiple sapA homologs are localized to a limited region on the chromosomal DNA of C. fetus and thus suggest the possibility of developing a typing system using this method.

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