Hepatitis C virus infection in hemodialysis patients: comparison of two new hepatitis C antibody assays with a second-generation assay
- PMID: 8589320
- DOI: 10.1681/ASN.V651439
Hepatitis C virus infection in hemodialysis patients: comparison of two new hepatitis C antibody assays with a second-generation assay
Abstract
The performance of two new hepatitis C virus antibody (anti-HCV) assays (a third-generation immunoglobulin (Ig)G recombinant immunoblot assay (RIBA 3.0) and hepatitis C virus core IgM (HCV IgM) in the prediction of hepatitis C viremia in hemodialysis patients was compared with that of a second-generation IgG recombinant immunoblot assay (RIBA 2.0). Forty-three patients on maintenance hemodialysis were studied. Aliquots of sera were tested prospectively for anti-HCV by RIBA 2.0, RIBA 3.0, and HCV IgM and for HCV RNA by polymerase chain reaction. Thirty-eight patients were HCV RNA positive. Among those, 7 (18%) were HCV IgM positive, 22 (58%) were RIBA 2.0 positive, and 29 (76%) were RIBA 3.0 positive. All but one viremic patients detected by HCV IgM were also detected by RIBA 2.0 and RIBA 3.0. All viremic patients detected by RIBA 2.0 were also detected by RIBA 3.0. RIBA 3.0 was more sensitive than RIBA 2.0 and HCV IgM in the detection of viremic patients (P = 0.0156 and < 0.0001, respectively). The positive predictive value for HCV IgM was 100% as compared with 96 and 97% for RIBA 2.0 and RIBA 3.0, respectively. The negative predictive value for RIBA 3.0 was 36% as compared with 24 and 14% for RIBA 2.0 and HCV IgM, respectively. At 6-months follow-up of the eight viremic patients without a detectable IgM or IgG anti-HCV response, all patients remained RIBA 2.0 nonreactive, one became RIBA 3.0 indeterminate, and one became HCV IgM positive. These data suggest that HCV IgM has poor sensitivity in the detection of hepatitis C viremia and RIBA 3.0 improves the sensitivity of IgG anti-HCV assays in the early detection of hepatitis C viremia in hemodialysis patients.
Similar articles
-
New insights into hepatitis C virus infection of hemodialysis patients: the implications.Am J Kidney Dis. 1995 Apr;25(4):572-8. doi: 10.1016/0272-6386(95)90125-6. Am J Kidney Dis. 1995. PMID: 7702052
-
Detection of anti-hepatitis C virus antibodies in patients undergoing dialysis by utilizing a hepatitis C virus 3.0 assay: correlation with hepatitis C virus RNA.J Lab Clin Med. 1998 Jul;132(1):73-5. doi: 10.1016/s0022-2143(98)90028-2. J Lab Clin Med. 1998. PMID: 9665375
-
HCV viremia in hemodialysis patients: detection by a DNA enzyme immunoassay for amplified HCV sequences.Ren Fail. 1995 Sep;17(5):565-73. doi: 10.3109/08860229509037621. Ren Fail. 1995. PMID: 8570869
-
[Anti-HCV serology for screening, diagnosis and surveillance of hepatitis C: role of the immunoblot].Ann Biol Clin (Paris). 1998 Jul-Aug;56(4):417-26. Ann Biol Clin (Paris). 1998. PMID: 9754277 Review. French.
-
Diagnostic tests for hepatitis C.Hepatology. 1997 Sep;26(3 Suppl 1):43S-47S. doi: 10.1002/hep.510260708. Hepatology. 1997. PMID: 9305663 Review.
Cited by
-
Gonococcal opacity: lectin-like interactions between Opa proteins and lipooligosaccharide.Infect Immun. 1995 Apr;63(4):1434-9. doi: 10.1128/iai.63.4.1434-1439.1995. Infect Immun. 1995. PMID: 7890406 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical