[Development of a sandwich ELISA for detection of specific antibodies against fimbrial antigens K88, K99 and 987p of Escherichia coli]

Abstract

For the assessment of the efficacy of a E. coli-vaccine it was important to detect the specific antibodies against the protective antigens. Under the use of monoclonal antibodies against the fimbrial antigens K88, K99 and 987p a sandwich-elisa was developed. With these elisa-tests we detected significant titre conversion after repeated immunisation of rabbits. The results are highly reproducible. That is why the elisa-tests are qualified for testing of vaccine batches. The use of an anti-pig-conjugate makes it possible to detect antibodies in colostrum and serum from pigs.