Caffeine inhibits Ca2+ uptake by subplasmalemmal calcium stores ('alveolar sacs') isolated from Paramecium cells

Abstract

Caffeine inhibits 45Ca2+ sequestration by subplasmalemmal calcium stores ('alveolar sacs') of low thapsigargicin sensitivity which we have isolated from the ciliated protozoan, Paramecium tetraurelia. Inhibition depends on caffeine concentration, with an IC50 of 31.8 mM. According to kinetic evaluation this is compatible with non-competitive inhibition of Ca2+ uptake, rather than with superimposed 45Ca2+ release during sequestration. It remains to be analysed whether this mechanism might be of possible relevance also for Ca2+-mediated activation in vivo in this or in any other secretory system. Such an effect could also operate indirectly, e.g., by Ca2+-release induction via sequestration inhibition. This is the first description of caffeine-mediated inhibition of Ca2+ uptake by calcium stores from a secretory system. Our data are compatible with some observations with sarcoplasmic reticulum from striated muscle fibers.