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Clinical Trial
. 1995 Nov-Dec;89(6):622-4.
doi: 10.1016/0035-9203(95)90416-6.

Detection of Leishmania in the blood of early kala-azar patients with the aid of the polymerase chain reaction

S Adhya  1 , M Chatterjee, M Q Hassan, S Mukherjee, S Sen
Affiliations
Clinical Trial

Detection of Leishmania in the blood of early kala-azar patients with the aid of the polymerase chain reaction

S Adhya et al. Trans R Soc Trop Med Hyg. 1995 Nov-Dec.

Abstract

Samples from 39 patients with symptoms suggestive of early visceral leishmaniasis were independently assayed by microscopy of tissue smears, enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) of blood deoxyribonucleic acid. Of these patients, 19 were confirmed as positive or negative by all 3 tests; 11 patients (28%) negative by smear were positive by ELISA and PCR; and 7 (18%) were positive by PCR alone. These results demonstrate the high sensitivity of the non-invasive PCR and, to a smaller extent, ELISA, in the early diagnosis of visceral leishmaniasis.

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