Time course of release of content of single vesicles in bovine chromaffin cells

Abstract

The time course of the spontaneous current spikes produced by release of the catecholamine contents of individual vesicles was examined in bovine chromaffin cells using carbon filament electrodes. The rate of spontaneous release was enhanced by adding either LaCl3 (0.01-0.5 mM) or BaCl2 (2 mM) to the extracellular solution. A paucity of events of very short duration was evident from the frequency histograms of the rise and the decay times. In the scatterograms of the rise and of the decay times the regression lines are invariably positive (i.e. the longer the duration of the rise times the longer the duration of the decay times). However, the regression lines never go through the origin but intercept the ordinate (the axis of the decay times) at (+/- SD) 16.1 +/- 6.4 ms (n = 11). On the other hand, the regression lines of paired rise and decay times for the time courses of diffusion are both linear and go through the origin. This relationship holds irrespective of whether the diffusion from an instantaneous point source was assumed to occur in an infinite plane or in an infinite volume. Therefore our experimental findings are incompatible with the model(s) assuming that diffusional broadening determines entirely the time course of current spikes. However, they can be explained, although only partially, by the possible slow speed of the electrode. They thus suggest that in chromaffin cells the duration of exocytosis of individual vesicles is much longer than in synapses.