Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1996 Apr 2;93(7):3132-7.
doi: 10.1073/pnas.93.7.3132.

An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae

W J Philipp  1 , S Poulet, K Eiglmeier, L Pascopella, V Balasubramanian, B Heym, S Bergh, B R Bloom, W R Jacobs Jr, S T Cole
Affiliations
Comparative Study

An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae

W J Philipp et al. Proc Natl Acad Sci U S A. .

Abstract

An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis, was constructed by using a twin-pronged approach. Pulsed-field gel electrophoretic analysis enabled cleavage sites for Asn I and Dra I to be positioned on the 4.4-Mb circular chromosome, while, in parallel, clones from two cosmid libraries were ordered into contigs by means of fingerprinting and hybridization mapping. The resultant contig map was readily correlated with the physical map of the genome via the landmarked restriction sites. Over 165 genes and markers were localized on the integrated map, thus enabling comparisons with the leprosy bacillus, Mycobacterium leprae, to be undertaken. Mycobacterial genomes appear to have evolved as mosaic structures since extended segments with conserved gene order and organization are interspersed with different flanking regions. Repetitive sequences and insertion elements are highly abundant in M. tuberculosis, but the distribution of IS6110 is apparently nonrandom.

PubMed Disclaimer

References

    1. J Bacteriol. 1993 Jun;175(11):3422-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6630-4 - PubMed
    1. Mol Microbiol. 1993 Jan;7(2):197-206 - PubMed
    1. FEMS Microbiol Rev. 1994 Jun;14(2):139-60 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Oct;83(20):7821-5 - PubMed

Publication types

Substances

Associated data

LinkOut - more resources