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Comparative Study
. 1996 May;3(5):439-45.
doi: 10.1038/nsb0596-439.

Packing interactions in the apomyglobin folding intermediate

M S Kay  1 , R L Baldwin
Affiliations
Comparative Study

Packing interactions in the apomyglobin folding intermediate

M S Kay et al. Nat Struct Biol. 1996 May.

Abstract

The contribution of specific packing to the stability of the sperm whale apomyoglobin intermediate has been studied by urea denaturation monitored by circular dichroism and fluorescence. Mutations disrupting native packing sites within the subdomain formed by the A, G and H helices destabilize the intermediate, in contrast to the conclusion drawn from earlier studies of pH-induced unfolding. Based on these results, the intermediate is proposed to be stabilized by both partially formed native-like tertiary, and non-specific hydrophobic interactions forming a subdomain folding intermediate. The results help to explain how the intermediate acquires its structure and stability.

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