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. 1996 Mar;87(3):269-74.
doi: 10.1111/j.1349-7006.1996.tb00216.x.

Establishment of an undifferentiated leukemia cell line (Kasumi-3) with t(3;7)(q27;q22) and activation of the EVI1 gene

H Asou  1 , K Suzukawa, K Kita, K Nakase, H Ueda, K Morishita, N Kamada
Affiliations

Establishment of an undifferentiated leukemia cell line (Kasumi-3) with t(3;7)(q27;q22) and activation of the EVI1 gene

H Asou et al. Jpn J Cancer Res. 1996 Mar.

Abstract

A novel human leukemia cell line (Kasumi-3) was established from the blast cells of a 57-year-old man suffering from myeloperoxidase-negative acute leukemia. The cell line had five distinctive features, as follows. 1) Flow cytometric analyses showed cell surface expression of CD7, CD4, CD13, CD33, CD34, HLA-DR and c-Kit. This phenotype is compatible with that of acute myelocytic leukemia cells with the M0 subtype in the French-American-British classification. 2) Kasumi-3 cells carried chromosomal abnormalities of t(3;7)(q27:q22), del(5)(q15), del(9)(q32), and add(12)(p11). The breakpoint of 3q27 was located near the EVI1 gene, and a high level of expression of the EVI1 gene was observed. 4) Kasumi-3 cells treated with TPA showed maturation to monocytic lineage. 5) Treatment with either interleukin (IL)-2, IL-3, IL-4, granulocyte-macrophage colony-stimulating or stem cell factor induced the proliferation of Kasumi-3 cells. Thus, the Kasumi-3 cell line shows the characteristic features of undifferentiated leukemia. It should, therefore, be useful both for studying the biological characteristics of acute myelogenous leukemia M0 subtype and for investigating the role of the EVI1 gene in leukemogenesis.

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References

    1. ) Lee , E. J. , Schiffer , C. A. , Tomiyasu , T. and Testa , J. R.Clinical and cytogenetic correlations of abnormal megakaryopoiesis in patients with acute leukemia and chronic myelogenous leukemia in blast crisis . Leukemia 4 , 350 – 353 ( 1990. ). - PubMed
    1. ) Fonatsch , C. , Gudat , H. , Lengfelder , E. , Wandt , H. , Silling‐Engrelhardt , C. , Ludwig , W. D. , Thiel , E. , Freund , M. , Bodenstein , H. , Schwieder , G. , Gruneisen , A. , Aul , C. , Schnittger , S. , Rieder , H. , Haase , D. and Hild , F.Correlation of cytogenetic findings with clinical features in 18 patients with inv(3)(q21q26) or t(3;3)(q21;q26) . Leukemia 8 , 1318 – 1326 ( 1994. ). - PubMed
    1. ) Bitter , M. A. , Neilly , M. E. , Le Beau , M. M. , Pearson , M. G. and Rowley , J. D.Rearrangements of chromosome 3 involving bands 3q21 and 3q26 are associated with normal or elevated platelet counts in acute nonlymphocytic leukemia . Blood 66 , 1362 – 1370 ( 1985. ). - PubMed
    1. ) Morishita , K. , Parganas , E. , Willman , C. L. , Whittaker , M. H. , Drabkin , H. , Oval , J. , Taetle , R. and Ihle , J. N.Activation of EVI1 gene expression in human acute myelogenous leukemias by translocations spanning 300–500 kilobases on chomosome 3q26 . Proc. Natl. Acad. Sci. USA 89 , 3937 – 3941 ( 1992. ). - PMC - PubMed
    1. ) Suzukawa , K. , Parganas , E. , Gajjar , A. , Abe , T. , Takahashi , S. , Tani , K. , Asano , S. , Asou , H. , Kamada , N. , Yokota , J. , Morishita , K. and Ihle , J. N.Identification of a breakpoint cluster region 3′ of the Ribophorin I associated with the transcriptional activation of the EVI1 gene in acute myelogenous leukemias with inv(3)(q21q26) . Blood 84 , 2681 – 2688 ( 1994. ). - PubMed

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