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Comparative Study
. 1996 Apr 16;93(8):3653-7.
doi: 10.1073/pnas.93.8.3653.

Human TOP3: a single-copy gene encoding DNA topoisomerase III

R Hanai  1 , P R Caron, J C Wang
Affiliations
Comparative Study

Human TOP3: a single-copy gene encoding DNA topoisomerase III

R Hanai et al. Proc Natl Acad Sci U S A. .

Abstract

A human cDNA encoding a protein homologous to the Escherichia coli DNA topoisomerase I subfamily of enzymes has been identified through cloning and sequencing. Expressing the cloned human cDNA in yeast (delta)top1 cells lacking endogenous DNA topoisomerase I yielded an activity in cell extracts that specifically reduces the number of supercoils in a highly negatively supercoiled DNA. On the basis of these results, the human gene containing the cDNA sequence has been denoted TOP3, and the protein it encodes has been denoted DNA topoisomerase III. Screening of a panel of human-rodent somatic hybrids and fluorescence in situ hybridization of cloned TOP3 genomic DNA to metaphase chromosomes indicate that human TOP3 is a single-copy gene located at chromosome 17p11.2-12.

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