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. 1977 Jun;130(3):1274-80.
doi: 10.1128/jb.130.3.1274-1280.1977.

Molecular sizes and relationships of TOL plasmids in Pseudomonas

C J Duggleby, S A Bayley, M J Worsey, P A Williams, P Broda

Molecular sizes and relationships of TOL plasmids in Pseudomonas

C J Duggleby et al. J Bacteriol. 1977 Jun.

Abstract

Plasmid deoxyribonucleic acid was isolated from thirteen Pseudomonas strains judged on genetic criteria to carry plasmids coding for the degradation of toluene and m- and p-xylenes (TOL plasmids). Most strains carried a single species, but two strains carried two size classes, and cells of a third strain contained plasmids ranging in size from 25 X 10(6) to 202 X 10(6) daltons. Some plasmids could be transformed into a Pseudomonas putida strain to yield Tol+ progeny. Plasmids from 5 of the 13 strains were indistinguishable on the basis of size and gel pattern of fragments after endonuclease digestion.

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