Nucleotide sequence of Thermus aquaticus ribosomal 5 S ribonucleic acid. Sequence homologies in thermophilic organisms

Abstract

The nucleotide sequence of ribosomal 5 S RNA from Thermus aquaticus grown at 75 degrees is p(A)-A-U-C-C-C-C-G-C-C-C-U-U-A-G-C-G-G-C-G-U-G-G-A-A-C-A-C-C-C-G-U-U-C-C-C-A-U-U-C-C-G-A-A-C-A-C-G-G-A-A-G-U-G-A-A-A-C-G-C-G-C-C-A-G-C-G-C-C-G-A-U-G-G-U-C-A-C-U-G-G-G-A-C-C-G-C-A-G-G-G-U-C-C-U-G-G-A-G-A-G-U-A-G-G-U-G-C-U-G-G-U-G-C-G-G-G-G-A-(U). The major molecular species is 120 nucleotides long; some molecules are one or two nucleotides shorter with one less nucleotide at either or both termini. When compared to other 5 S rRNAs, the sequence homology was greater with a thermophile bacterium (Bacillus stearothermophilus) than with mesophilic species. The comparisons further indicate that among prokaryotes, eleven of the nucleotide residues in T. aquaticus 5 S RNA may be largely restricted to thermophiles. Possible models for the secondary structure of T. aquaticus 5 S rRNA are discussed with respect to products of limited digestion and the unique nucleotides.