Altered molecular species of human interferon produced in the presence of inhibitors of glycosylation
- PMID: 863934
Altered molecular species of human interferon produced in the presence of inhibitors of glycosylation
Abstract
The inhibitors of glycosylation, 2-deoxy-D-glucose or D-glucosamine, inhibit the synthesis of biologically active interferon in human FS-4 fibroblast cultures stimulated with polyinosinate-polycytidylate. Interferon synthesized in the presence of partially inhibitory concentrations of 2-deoxy-D-glucose or D-glucosamine were found to differ from interferons made in control cultures in some physical properties. Interferons synthesized in the presence of either inhibitor had a diminished charge heterogeneity demonstrable by iso-electric focusing. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis, control interferon activity formed a single peak with the apparent molecular weight of 20,000, whereas interferons from cultures treated with either inhibitor could be resolved into two distinct molecular weight components, one of which was smaller than the interferon synthesized in control cultures.
Similar articles
-
The effect of inhibitors of glycosylation on interferon production in human lymphoblastoid cells.J Gen Virol. 1979 May;43(2):457-61. doi: 10.1099/0022-1317-43-2-457. J Gen Virol. 1979. PMID: 479846
-
Effect of glycosylation inhibitors on the production and properties of human leukocyte interferon.Virology. 1979 Sep;97(2):473-6. doi: 10.1016/0042-6822(79)90359-3. Virology. 1979. PMID: 224593 No abstract available.
-
Suppression of human interferon production by inhibitors of glycosylation.Virology. 1975 Feb;63(2):475-83. doi: 10.1016/0042-6822(75)90320-7. Virology. 1975. PMID: 1168006 No abstract available.
-
Interferoids: in vitro and in vivo conversion of native interferons to lower molecular weight forms.Proc Natl Acad Sci U S A. 1978 Oct;75(10):4814-8. doi: 10.1073/pnas.75.10.4814. Proc Natl Acad Sci U S A. 1978. PMID: 217001 Free PMC article.
-
Inhibition of the multiplication of enveloped viruses by glucose derivatives.Curr Top Microbiol Immunol. 1975;70:101-19. doi: 10.1007/978-3-642-66101-3_4. Curr Top Microbiol Immunol. 1975. PMID: 808397 Review. No abstract available.
Cited by
-
Constitutive, long-term production of human interferons by hamster cells containing multiple copies of a cloned interferon gene.Nucleic Acids Res. 1983 Feb 11;11(3):687-706. doi: 10.1093/nar/11.3.687. Nucleic Acids Res. 1983. PMID: 6188107 Free PMC article.
-
Elimination of size and charge heterogeneities of human leukocyte interferons by chemical cleavage.Proc Natl Acad Sci U S A. 1977 Oct;74(10):4200-4. doi: 10.1073/pnas.74.10.4200. Proc Natl Acad Sci U S A. 1977. PMID: 200907 Free PMC article.
-
Evidence for a unique human fibroblast interferon (IFN-beta 1) chromosomal gene, devoid of intervening sequences.Nucleic Acids Res. 1981 Feb 11;9(3):461-71. doi: 10.1093/nar/9.3.461. Nucleic Acids Res. 1981. PMID: 6164047 Free PMC article.
-
Differences between human alpha (leukocyte) and beta (fibroblast) interferons.Arch Virol. 1981;67(4):267-81. doi: 10.1007/BF01314831. Arch Virol. 1981. PMID: 6165341 Review. No abstract available.
-
The glycoprotein nature of murine gamma interferon.Arch Virol. 1984;80(2-3):195-207. doi: 10.1007/BF01310659. Arch Virol. 1984. PMID: 6202280
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
