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. 1995:(33):224-5.

The branch site adenosine is recognized differently for the two steps of pre-mRNA splicing

C C Query  1 , S A Strobel, P A Sharp
Affiliations
  • PMID: 8643377

The branch site adenosine is recognized differently for the two steps of pre-mRNA splicing

C C Query et al. Nucleic Acids Symp Ser. 1995.

Abstract

The functional groups responsible for branch site identity in the two steps of pre-mRNA splicing as well as for spliceosome assembly were tested by incorporation of site-specific modifications at the branch site of a pre-mRNA. These results show that recognition of the adenosine occurs early in complex formation and that the branch site adenosine is recognized differently before the first step and for the second step. Further, direct UV cross-linking with these modified RNAs was used to identify a factor whose interaction was dependent upon the identity of the branch site nucleotide.

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