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. 1996 May 28;93(11):5203-7.
doi: 10.1073/pnas.93.11.5203.

Quantal acetylcholine release induced by mediatophore transfection

J Falk-Vairant  1 , P Corrèges, L Eder-Colli, N Salem, E Roulet, A Bloc, F Meunier, B Lesbats, F Loctin, M Synguelakis, M Israel, Y Dunant
Affiliations

Quantal acetylcholine release induced by mediatophore transfection

J Falk-Vairant et al. Proc Natl Acad Sci U S A. .

Abstract

Mediatophore is a protein of approximately 200 kDa able to translocate acetylcholine in response to calcium. It was purified from the presynaptic plasma membranes of the electric organ nerve terminals. Mediatophore is a homooligomer of a 16-kDa subunit, homologous to the proteolipid of V-ATPase. Cells of the N18TG-2 neuronal line are not able to produce quantal acetylcholine release. We show here that transfection of N18TG-2 cells with a plasmid encoding the mediatophore subunit restored calcium-dependent release. The essential feature of such a release was its quantal nature, similar to what is observed in situ in cholinergic synapses from which mediatophore was purified.

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