Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 Oct 15;67(20):3802-5.
doi: 10.1021/ac00116a030.

Attomole-sensitivity electrospray source for large-molecule mass spectrometry

G A Valaskovic  1 , N L Kelleher, D P Little, D J Aaserud, F W McLafferty
Affiliations

Attomole-sensitivity electrospray source for large-molecule mass spectrometry

G A Valaskovic et al. Anal Chem. .

Abstract

Full mass spectra of high resolving power are obtained from 0.2 nL sample volumes of large (> 10 kDa) nucleotides and proteins using a new electrospray ionization (ESI) system combined with Fourier transform mass spectrometry. The ESI needles are fabricated by laser-heated pulling of fused-silica tubing (5-20 microns i.d.), followed by chemical etching and surface metalization. Total analyte loaded at the instrument of 8.6 fmol and 216 amol produces signal-to-noise ratios of 400:1 and 60:1, respectively, and resolving power of > 10(5) for full mass spectra, while the total amount of material consumed is approximately 150 and 10 amol, respectively.

PubMed Disclaimer

Publication types

LinkOut - more resources