Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 May;22(2):520-6.
doi: 10.1128/JVI.22.2.520-526.1977.

Sequence analysis of the nicks and termini of bacteriophage T5 DNA

B P Nichols, J E Donelson

Sequence analysis of the nicks and termini of bacteriophage T5 DNA

B P Nichols et al. J Virol. 1977 May.

Abstract

Bacteriophage T5 DNA, when isolated from mature phage particles, contains several nicks in one of the two strands. The 5'-terminal nucleotides at the nicks were labeled with polynucleotide kinase and [gamma-32P]ATP, and the 3'-terminal nucleotides were labeled with Escherichia coli DNA polymerase I and [alpha-32P]dGTP. The sequences around the nicks were analyzed by partial nuclease digestion followed by homochromatography fractionation of the resulting oligonucleotides. The nicks had at least the sequence -PuOH pGpCpGpC- in common. In addition, the two 5' external termini had the first seven nucleotides in common.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. J Virol. 1977 May;22(2):510-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 May;71(5):2108-12 - PubMed
    1. J Mol Biol. 1972 Feb 28;64(1):87-102 - PubMed
    1. Science. 1969 Mar 28;163(3874):1410-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 May;73(5):1576-80 - PubMed

Publication types

LinkOut - more resources