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. 1977 May;22(2):520-6.
doi: 10.1128/JVI.22.2.520-526.1977.

Sequence analysis of the nicks and termini of bacteriophage T5 DNA

B P Nichols, J E Donelson

Sequence analysis of the nicks and termini of bacteriophage T5 DNA

B P Nichols et al. J Virol. 1977 May.

Abstract

Bacteriophage T5 DNA, when isolated from mature phage particles, contains several nicks in one of the two strands. The 5'-terminal nucleotides at the nicks were labeled with polynucleotide kinase and [gamma-32P]ATP, and the 3'-terminal nucleotides were labeled with Escherichia coli DNA polymerase I and [alpha-32P]dGTP. The sequences around the nicks were analyzed by partial nuclease digestion followed by homochromatography fractionation of the resulting oligonucleotides. The nicks had at least the sequence -PuOH pGpCpGpC- in common. In addition, the two 5' external termini had the first seven nucleotides in common.

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References

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