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. 1996 Jun;70(6):4146-9.
doi: 10.1128/JVI.70.6.4146-4149.1996.

Maintenance of pluripotency in mouse embryonic stem cells persistently infected with murine coronavirus

A Okumura  1 , K Machii, S Azuma, Y Toyoda, S Kyuwa
Affiliations

Maintenance of pluripotency in mouse embryonic stem cells persistently infected with murine coronavirus

A Okumura et al. J Virol. 1996 Jun.

Abstract

A persistently coronavirus-infected embryonic stem (ES) cell line A3/MHV was established by infecting an ES cell line, A3-1, with mouse hepatitis virus type-2. Although almost all A3/MHV cells were found infected, both A3/MHV and A3-1 cells expressed comparable levels of cell surface differentiation markers. In addition, A3/MHV cells retained the ability to form embryoid bodies. These results suggest that persistent coronavirus infection does not affect the differentiation of ES cells.

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