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. 1996 May 1;24(9):1608-15.
doi: 10.1093/nar/24.9.1608.

The polypyrimidine tract binding (PTB) protein interacts with single-stranded DNA in a sequence-specific manner

F Brunel  1 , M M Zakin, H Buc, M Buckle
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The polypyrimidine tract binding (PTB) protein interacts with single-stranded DNA in a sequence-specific manner

F Brunel et al. Nucleic Acids Res. .

Abstract

Polypyrimidine tract binding (PTB) protein is a cellular factor whose function is unknown. Various RNA or single-stranded DNA sequences have been shown to interact with PTB. In this paper, using laser UV crosslinking and electrophoretic mobility shift assays to probe DNA-protein interactions, we demonstrate that PTB binding at a single-stranded DNA target is highly sequence-specific. We provide data showing that PTB interacts with the top strand of the adenovirus major late promoter transcriptional initiator, a sequence rich in pyrimidine residues. We also demonstrate that PTB is organised into at least two different binding domains.

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