Detection of Helicobacter pylori infection by saliva IgG testing
- PMID: 8651161
Detection of Helicobacter pylori infection by saliva IgG testing
Abstract
Objectives: Most currently available tests for the detection of Helicobacter pylori are invasive, time consuming, or impractical. We examined the test performance of a simple and rapidly administered salivary IgG assay kit in the diagnosis of Helicobacter pylori infection.
Methods: Patients referred to a tertiary care setting for upper gastrointestinal endoscopy were included in a prospective evaluation of the test performance of the Helisal Kit which uses an ELISA technique to determine IgG antibodies in saliva. The results of the salivary IgG assay were compared to those of the Helisal Serum Kit, and to gastric histology. Two by two contingency table analyses were performed, and 95% confidence intervals (CI) were determined.
Results: Upper gastrointestinal endoscopy was performed on 106 patients over a 3-month period. A statistically significant correlation was found between the blood and saliva IgG results (r = 0.60, p = 0.0001). When compared to serum IgG, the salivary assay test performance was: sensitivity 84% (CI: 70-93%), specificity 81% (CI: 69-90%), positive predictive value 76% (CI: 61-87%), negative predictive value 88% (CI: 76-95%), and diagnostic accuracy 88% (CI: 76-95%). Compared with gastric histology, the test performance of the salivary IgG assay decreased to: sensitivity 66% (CI: 52-79%), specificity 74% (CI: 60-85%), positive predictive value 71% (CI: 57-83%), negative predictive value 68% (CI: 55-80%), and diagnostic accuracy 70% (CI: 60-78%). More specifically, the salivary assay gave false-negative results in nine of 17 patients with duodenal ulcers. Results did not vary significantly when outcomes of the salivary and serum assays were combined. The incremental information obtained in the salivary test was greatest in the patient population exhibiting an intermediate pretest probability (30-70%) of being infected with Helicobacter.
Conclusion: The salivary IgG results correlated significantly with the serum IgG titers but exhibited only modest test performance, compared with the results of gastric histology. This salivary test may be most useful in certain patient subpopulations or in specific clinical contexts.
Similar articles
-
The effectiveness of (IgG-ELISA) serology as an alternative diagnostic method for detecting Helicobacter pylori infection in patients with gastro-intestinal bleeding due to gastro-duodenal ulcer.Rev Esp Enferm Dig. 2002 Dec;94(12):725-36. Rev Esp Enferm Dig. 2002. PMID: 12733331 English, Spanish.
-
Salivary-specific immunoglobulin G in the diagnosis of Helicobacter pylori infection in dyspeptic patients.Am J Gastroenterol. 1995 Oct;90(10):1820-3. Am J Gastroenterol. 1995. PMID: 7572901
-
Salivary IgG assay to detect Helicobacter pylori infection in an Indian adult population.Indian J Dent Res. 2012 Sep-Oct;23(5):694-5. doi: 10.4103/0970-9290.107416. Indian J Dent Res. 2012. PMID: 23422628
-
[ELISA tests for detection of Helicobacter pylori antibodies].Nihon Rinsho. 1993 Dec;51(12):3201-4. Nihon Rinsho. 1993. PMID: 8283633 Review. Japanese.
-
Accuracy of diagnostic methods used for epidemiological studies of Helicobacter pylori.Aliment Pharmacol Ther. 1995;9 Suppl 2:21-31. Aliment Pharmacol Ther. 1995. PMID: 8547524 Review.
Cited by
-
Evaluation of commercially available Helicobacter pylori serology kits: a review.J Clin Microbiol. 1998 Oct;36(10):2803-9. doi: 10.1128/JCM.36.10.2803-2809.1998. J Clin Microbiol. 1998. PMID: 9738024 Free PMC article. Review. No abstract available.
-
Performance characteristics of an enzyme-linked immunosorbent assay for determining salivary immunoglobulin G response to Helicobacter pylori.J Clin Microbiol. 1999 Feb;37(2):430-2. doi: 10.1128/JCM.37.2.430-432.1999. J Clin Microbiol. 1999. PMID: 9889234 Free PMC article.
-
Non-invasive diagnostic tests for Helicobacter pylori infection.Cochrane Database Syst Rev. 2018 Mar 15;3(3):CD012080. doi: 10.1002/14651858.CD012080.pub2. Cochrane Database Syst Rev. 2018. PMID: 29543326 Free PMC article.
-
Helicobacter pylori detection and antimicrobial susceptibility testing.Clin Microbiol Rev. 2007 Apr;20(2):280-322. doi: 10.1128/CMR.00033-06. Clin Microbiol Rev. 2007. PMID: 17428887 Free PMC article. Review.
-
Evaluation of a commercial serological kit for detection of salivary immunoglobulin G to Helicobacter pylori.Eur J Clin Microbiol Infect Dis. 1997 Oct;16(10):775-7. doi: 10.1007/BF01709266. Eur J Clin Microbiol Infect Dis. 1997. PMID: 9405955 No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Medical