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. 1996 Jun 3;1307(1):31-4.
doi: 10.1016/0167-4781(96)00057-7.

Cloning of a cDNA encoding a human homologue of CDC47, a member of the MCM family

T Kiyono  1 , M Fujita, Y Hayashi, M Ishibashi
Affiliations

Cloning of a cDNA encoding a human homologue of CDC47, a member of the MCM family

T Kiyono et al. Biochim Biophys Acta. .

Abstract

A complementary DNA (cDNA) clone encoding a 719-amino acid (aa) protein was isolated, which has a 49% aa identity with budding yeast CDC47, a member of the MCM family. Antiserum raised against a C-terminal polypeptide bacterially produced from the cDNA clone detected a cellular protein about 80 kDa, which coincides with the size of the in vitro transcription/translation product of the cDNA. These results indicate that the cDNA covers the entire coding region of a human homologue of CDC47.

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