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. 1996 Jun 3;1307(1):55-65.
doi: 10.1016/0167-4781(95)00218-9.

Complex interactions with direct repeats of a mitogen-responsive VL30 enhancer

D Pribnow  1 , S L Chen, Y Zhang, B E Magun
Affiliations

Complex interactions with direct repeats of a mitogen-responsive VL30 enhancer

D Pribnow et al. Biochim Biophys Acta. .

Abstract

The RVL-3 VL30 enhancer is an LTR-derived triple direct repeat of 35 base pairs that mediates gene induction in response to several different intracellular signaling pathways. Using mobility shift assays, methylation interference and DNase I footprinting, we have investigated the physical interactions between the RVL-3 enhancer and components of nuclear extracts from Rat-1 cells. Each enhancer repeat unit contains a single binding site. Our studies suggest that binding to the double or triple repeat enhancer is cooperative, involving simultaneous occupation of two sites, with a preference for adjacent sites. Binding cooperativity would have implications for the mechanism of gene activation directed from the native VL30 enhancer.

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