Thiamine pyrophosphate as an effector of 2-oxoglutarate dehydrogenase complex from European bison heart
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- PMID: 8653071
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Thiamine pyrophosphate as an effector of 2-oxoglutarate dehydrogenase complex from European bison heart
Biochem Mol Biol Int.
1995 Sep.
Abstract
The purified 2-oxoglutarate dehydrogenase complex (OGDC) from the European bison heart was near saturated with endogenous bound thiamine pyrophosphate (TPP). Exogenous TPP added to the full OGDC reaction medium decreased S0.5 for 2-oxoglutarate approximately 2.6-fold without any notable change in the maximum reaction rate. The TPP effect was observed in the presence of 1 mM ADP which alone is a strong positive allosteric effector of OGDC. At an unsaturating 2-oxoglutarate concentration the A50 value for TPP was approximately 0.05 mM. The ADP-like action of exogenous TPP was also found in the 2-oxoglutarate dehydrogenase (E1) reaction, determined in the presence of 2,6-dichlorophenoloindophenol as an electron acceptor.
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