Cloning, sequence determination and functional expression of the genes encoding adenovirus type-4 polymerase and the terminal protein precursor

Abstract

Sequences of the open reading frames encoding adenovirus type 4 (Ad4) DNA polymerase and the terminal protein precursor were determined. Sequence comparisons with the corresponding genes and proteins from Ad2 and Ad5 show high overall identity, but significant differences in those portions of the two proteins thought to be essential for their biological activities. Both Ad4 proteins were functionally expressed in insect cells from the corresponding cDNAs.