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. 1977 Mar;4(3):641-8.
doi: 10.1093/nar/4.3.641.

Endogenous DNA-directed DNA synthesising system in a microsomal fraction of embryonic chick brain

J Smith, L Soriano
Free PMC article

Endogenous DNA-directed DNA synthesising system in a microsomal fraction of embryonic chick brain

J Smith et al. Nucleic Acids Res. 1977 Mar.
Free PMC article

Abstract

A DNA polymerising complex directed by endogenous DNA has been partially purified from 11-day-old embryonic chick brain microsomes by DEAE-cellulose and phosphocellulose column chromatography. The active fractions are eluted together with an exogenous DNA-directed DNA polymerase; after Sephadex gel filtration, the endogenous activity remains associated with a high molecular weight DNA-directed DNA polymerase. The endogenous activity of the complex has been shown to be RNase-resistant and actinomycin-sensitive. It requires potassium, an ATP-regenerating system and all four deoxyribonucleoside triphosphates for full activity. The significance of this activity with regard to the protovirus hypothesis is discussed.

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