doi: 10.1093/nar/4.4.1135.
Rapid synthesis of oligodeoxyribonucleotides: a new solid-phase method
- PMID: 866189
- PMCID: PMC342510
- DOI: 10.1093/nar/4.4.1135
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Rapid synthesis of oligodeoxyribonucleotides: a new solid-phase method
Nucleic Acids Res.
1977 Apr.
Free PMC article
Abstract
A method is described that makes use of a new polyamide resin for the rapid synthesis of short oligodeoxyribonucleotides. The method is illustrated by the preparation of two heptadeoxyribonucleotides, d(pT6-C) and d(pC-A-G-T-G-A-T) using a phosphodiester approach. A further development involved use of phenyl isocyanate as an in situ drying agent, which obviated the need for solvent co-evaporation prior tothe internucleotidic coupling steps. Improved fractionation of thymidyl oligonucleotides was obtained by use of a new microparticulate, silica-based anion-exchanger.
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