Polylysine titration of rat liver chromatin fractions after DNase II digestion

Abstract

The concentration of free phosphate groups is measured in rat liver chromatin after DNase II digestion using polylysine titration. The unsheared chromatin completely precipitates at lysine/DNA phosphate ratios of 0.5 to 0.6. Digestion of the chromatin reduces the lysine/DNA phosphate ratio of complete precipitation by about 0.2 units suggesting the removal of free phosphate groups. The two chromatin fractions: MgC12 insoluble (template-inactive) and Mg12 soluble (template-active) chromatins precipitate at about the same lysine/DNA phosphate ratio. Some 15% of the MgC12 soluble chromatin remains in solution at any polylysine concentration. The removal of histone H 1 FROM THE MgC12 insoluble chromatin increases the lysine/DNA phosphate ratio by about 0.2 units suggesting that 20% of the DNA phosphate groups in nucleosomes are masked by histone H 1.