Salt-induced structural changes in nucleosomes
- PMID: 866195
- PMCID: PMC342489
- DOI: 10.1093/nar/4.4.853
Salt-induced structural changes in nucleosomes
Abstract
Nucleosomes and oligonucleosomes were prepared by digestion of human placental nuclei with staphlococcal nuclease and fractionated by gel filtration chromatography. The effect of increasing salt on the structure of nucleosomes was examined in the presence and absence of 10 mM MgCl2. Nucleosomes and oligonucleosomes are insoluble over a broad range of salt concentration. Nucleosomes are insoluble in larger than or equal to 120 mM (NH4)2SO4 containing 10 mM MgCl2 allowing analyses of changes in nucleosomal DNA by C.D. spectroscopy. Nucleosomes are insoluble in less than or equal to 120 mM (NH4)2SO4 containing 10 mM MgCl2 as demonstrated by turbidity measurements. We conclude that the insolubility of nucleosomes accompanies salt-induced structural changes possibly due to individual particle condensation. As the salt concentration is increased the nucleosomes condense and then relax at higher salt concentrations.
Similar articles
-
Lack of nucleosomal structure in a DNase-I-solubilized transcriptionally active chromatin fraction of Physarum polycephalum.Eur J Biochem. 1985 Mar 15;147(3):575-80. doi: 10.1111/j.0014-2956.1985.00575.x. Eur J Biochem. 1985. PMID: 3979388
-
Fractionation of micrococcal nuclease-digested chromatin solubilized at physiologic ionic strength.Exp Cell Res. 1985 Feb;156(2):563-9. doi: 10.1016/0014-4827(85)90564-6. Exp Cell Res. 1985. PMID: 3967693
-
Chicken erythrocyte nucleus contains two classes of chromatin that differ in micrococcal nuclease susceptibility and solubility at physiological ionic strength.Proc Natl Acad Sci U S A. 1981 Oct;78(10):5968-72. doi: 10.1073/pnas.78.10.5968. Proc Natl Acad Sci U S A. 1981. PMID: 6947211 Free PMC article.
-
The organization of oligonucleosomes in yeast.Nucleic Acids Res. 1983 Jun 11;11(11):3717-36. doi: 10.1093/nar/11.11.3717. Nucleic Acids Res. 1983. PMID: 6344013 Free PMC article.
-
Distribution of H1 histone in chromatin digested by micrococcal nuclease.Nucleic Acids Res. 1977 Oct;4(10):3281-301. doi: 10.1093/nar/4.10.3281. Nucleic Acids Res. 1977. PMID: 928061 Free PMC article.
Cited by
-
The sv40 transcription complex. I. Effect of viral chromatin proteins on endogenous RNA polymerase activity.Nucleic Acids Res. 1977 Dec;4(12):4261-77. doi: 10.1093/nar/4.12.4261. Nucleic Acids Res. 1977. PMID: 202928 Free PMC article.
-
Conformational changes of the chromatin subunit.Proc Natl Acad Sci U S A. 1978 Feb;75(2):660-3. doi: 10.1073/pnas.75.2.660. Proc Natl Acad Sci U S A. 1978. PMID: 416437 Free PMC article.
-
Isolation and characterization of a spacerless dinucleosome from H1-deleted chromatin.Nucleic Acids Res. 1977 Dec;4(12):4077-89. doi: 10.1093/nar/4.12.4077. Nucleic Acids Res. 1977. PMID: 600791 Free PMC article.
-
Use of RNA polymerase as an enzymatic probe of nucleosomal structure.Nucleic Acids Res. 1980 Sep 11;8(17):3851-64. doi: 10.1093/nar/8.17.3851. Nucleic Acids Res. 1980. PMID: 7003538 Free PMC article.
-
Responses of mammalian metaphase chromosomes to endonuclease digestion.Chromosoma. 1978 Dec 6;69(3):331-8. doi: 10.1007/BF00332136. Chromosoma. 1978. PMID: 743903
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
