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. 1977 Apr;4(4):899-915.
doi: 10.1093/nar/4.4.899.

Characterization of mRNA-protein complexes from mammalian cells

B Sundquist, T Persson, U Lindberg
Free PMC article

Characterization of mRNA-protein complexes from mammalian cells

B Sundquist et al. Nucleic Acids Res. 1977 Apr.
Free PMC article

Abstract

In a previous report we described the use of oligo(dT)-cellulose for the isolation of mRNA-protein complexes from EDTA-dissociated polysomes extracted from normally growing or adenovirus infected KB-cells (I). Experiments presented here provide evidence that proteins involved in these complexes bind specifically to mRNA since: a) the proteins and mRNA cosediment through sucrose gradients, b) they adsorb and elute from oligo(dT)-cellulose together, and c) analysis of the products from ribonuclease digestion experiments show that the poly (A) end and a separate small fraction of the mRNA are resistant to the enzymes and attached to protein.

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