Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Apr;4(4):917-28.
doi: 10.1093/nar/4.4.917.

Effect of homopolyribonucleotides on messenger ribonucleoprotein particles

B Sundquist, T Persson
Free PMC article

Effect of homopolyribonucleotides on messenger ribonucleoprotein particles

B Sundquist et al. Nucleic Acids Res. 1977 Apr.
Free PMC article

Abstract

A discrete set of polypeptides copurify with and appear to be specifically attached to mRNA from polysomes of eukaryotic cells. This report describes the effect of homopolyribonucleotides on mRNA-protein complexes separated from ribosome subunits by oligo(dT)-cellulose chromatography. It is shown that poly (U) and poly (A) can release mRNA-protein complexes adsorbed to oligo(dT)-cellulose, whereas poly (C) and poly(I) are much less effective in this process. Analysis of polyribonucleotide released material showed that poly(U) effectively dissociated the mRNA-protein complexes while poly(A) caused no or only partial derangement of these particles. The specificities seen in the polyribonucleotide effects in turn suggest a high degree of specificity in the interaction between the proteins and mRNA.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Ser Haematol. 1971;4(3):37-69 - PubMed
    1. J Virol. 1972 Nov;10(5):909-19 - PubMed
    1. Cell. 1975 Oct;6(2):185-95 - PubMed
    1. J Mol Biol. 1974 May 5;85(1):87-101 - PubMed
    1. Biochim Biophys Acta. 1975 Jan 20;378(2):251-9 - PubMed