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. 1996 Jul 26;273(5274):513-6.
doi: 10.1126/science.273.5274.513.

Persistent site-specific remodeling of a nucleosome array by transient action of the SWI/SNF complex

T Owen-Hughes  1 , R T Utley, J Côté, C L Peterson, J L Workman
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Persistent site-specific remodeling of a nucleosome array by transient action of the SWI/SNF complex

T Owen-Hughes et al. Science. .

Abstract

The SWI/SNF complex participates in the restructuring of chromatin for transcription. The function of the yeast SWI/SNF complex in the remodeling of a nucleosome array has now been analyzed in vitro. Binding of the purified SWI/SNF complex to a nucleosome array disrupted multiple nucleosomes in an adenosine triphosphate-dependent reaction. However, removal of SWI/SNF left a deoxyribonuclease I-hypersensitive site specifically at a nucleosome that was bound by derivatives of the transcription factor Gal4p. Analysis of individual nucleosomes revealed that the SWI/SNF complex catalyzed eviction of histones from the Gal4-bound nucleosomes. Thus, the transient action of the SWI/SNF complex facilitated irreversible disruption of transcription factor-bound nucleosomes.

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