[Hydroxylation of indolyl-3-acetic acid by the fungus aspergillus niger IBFM-F-12]

Abstract

Physiological and biochemical properties of the culture of Aspergillus niger IBPM F 12 carrying out hydroxylation IAA in the 4-, 5-, 6-positions of the indole nucleus were studied. The optimal composition of the medium for the cultivation was established. The transformation was performed by the washed fungal mycelium taken in the middle of the growth log-phase at a substrate concentration of I g/l. The correlation between the hydroxylase activity and pH, temperaure and biomass quantity was shown. The method of isolating 4-, 5- and 6-hydroxyindolyl-3-acetic acids in preparative amounts was developed.