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Clinical Trial
. 1995;36(4):499-508.
doi: 10.1186/BF03547664.

Hematological and blood biochemical effects of fasting and subsequent oral administration of endotoxin in prepubertal gifts

H Holst  1 , H Kindahl
Affiliations
Clinical Trial

Hematological and blood biochemical effects of fasting and subsequent oral administration of endotoxin in prepubertal gifts

H Holst et al. Acta Vet Scand. 1995.

Abstract
in English, German

The main objective of the present study was to investigate the effects of short-term fasting in gilts on endocrinological and blood biochemical parameters and, further, the effects of subsequent oral endotoxin (ET) administration. Group 1 was fasted for 30 h and then received feed with ET added. Group 2 was fasted for 30 h but received standard feed at refeeding. In group 3, gilts were fed every 6 h for 30 h. The major effects of fasting were: gradually increased concentration of plasma prostaglandin F2 alpha metabolite, serum total bilirubin, serum free fatty acids, and decreased serum glucose. The values were normalized within 1-4 h of refeeding. Twelve hours after refeeding, the ET-refed gilts showed higher levels of serum total bile acids and polymorphonuclear leukocytes than those in group 2. It is possible that the observed changes during fasting reflect either an increased intestinal uptake of naturally present endotoxin or a reduced endotoxin detoxifying capacity of the liver. The increased bile acid concentration and polymorphonuclear leukocyte count following refeeding with ET-feed may indicate that orally administered ET is to some extent absorbed from the gut.

Permanenta venkatetrar inopererades på tolv icke könsmogna gyltor av treraskorening (28–38 kg kroppsvikt) en vecka före försökets början. Djuren delades in i 3 grupper (n = 4). Gyltorna i grupp 1 fastades i 30 timmar varefter de utfodrades med foder med tillsats av endotoxin (Escherichia coli; 100 mg per djur). Grupp 2 fastades också i 30 timmar men erhöll vanligt foder vid återutfodring. Djuren i den tredje gruppen utfodrades, under motsvarande 30 timmar, var sjätte timme och tjänade därigenom som kontroller till de andra djuren under fasteintervall et. Blodprover togs regelbundet under fasteperioden, samt i 24 timmar efter återutfodring (ej grupp 3).

På de fastade djuren uppmättes bl.a. gradvis stigande blodkoncentrationer av prostaglandin F2α-metabolit, fria fettsyror och totalbilirubin. Värdena normaliserades inom några timmar efter återutfodring. Efter utfodring uppvisade de gyltor som endotoxinutfödrats högre blodkoncentrationer av gallsyror och polymorfkärniga leukocyter än de normalutfodrade djuren. Det är möjligt att de förhöjda koncentrationerna av prostaglandin F2α-metabolit under fasta orsakas av antingen en förändrad tunntarmspermeabilitet och därigenom en ökat upptag av i tarmen naturligt förekommande endotoxin, eller en försämrad endotoxindetoxifierande kapacitet i levern. De uppmätta forändringarna efter endotoxin utfodring talar för att oralt administrerat endotoxin, efter fasta, i viss utsträckning tas upp från magtarmkanalen till portablodet.

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